Pepsin hmoov CAS 9001-75-6

Lub luag haujlwm ntawm pepsin yog dab tsi? Qhia pepsin

Pepsin hmoovyog ib qho tseem ceeb heev digestive protease nyob rau hauv lub cev, uas yog tsuas yog secreted los ntawm lub ntsiab cell ntawm gastric mucosa. Cov zaub mov yuav tsum tau digested thiab decomposed nyob rau hauv lub plab ua hauj lwm thiab ces absorbed los ntawm lub cev. Lub plab mucosa secretes ntau pepsin los pab kev zom zaub mov, uas yuav tsum tau stimulation ntawm gastric acid los tsim cov protein. Pepsin tsuas tuaj yeem ua nws lub luag haujlwm hauv ib puag ncig acidic. Qhov siab dua tus nqi pH, qhov kev ua haujlwm ntawm pepsin yog qhov phem dua.Pepsin hauv plab ntawm cov neeg mob plab hnyuv thiab pernicious anemia feem ntau tsis zais txhua.

Pepsin yog secreted los ntawm lub ntsiab hlwb nyob rau hauv lub fundus ntawm lub plab, thiab nws yuav tsum tau mus ua si lub luag hauj lwm nyob rau hauv qhov kev txiav txim ntawm gastric acid. Clinically, kev ntsuam xyuas ntawm pepsin tuaj yeem kuaj xyuas kab mob plab, uas muaj qhov tseem ceeb hauv kev kho mob. Cov neeg mob uas muaj me ntsis pepsin secretion yuav raug mob plab hnyuv, atrophic gastritis, mob gastritis, pernicious anemia thiab lwm yam kab mob.

Cov txheej txheem ua kom Pepsin yog dab tsi?

Pepsin hmoovyog thawj zaug hais tias zymogen, uas yog, pepsinogen. Pepsinogen yog ib qho tsis muaj zog ua ntej ntawm pepsin, thiab nws cov qauv tseem ceeb muaj 44 amino acids ntau dua pepsin. Nyob rau hauv lub plab, pepsinogen raug tso tawm los ntawm lub ntsiab hlwb ntawm gastric mucosa. Qhov no proenzyme yog qhib thaum nws ua tau raws li hydrochloric acid nyob rau hauv gastric acid (tso tawm los ntawm gastric parietal hlwb). Thaum lub plab zom zaub mov, pepsinogen thiab hydrochloric acid raug tso tawm los ntawm lub plab phab ntsa raws li kev ua ntawm cov tshuaj hormones hu ua gastrin thiab vagus paj. Nyob rau hauv ib puag ncig acidic tsim los ntawm hydrochloric acid, pepsinogen yog nthuav tawm, yog li nws tuaj yeem siv los ua
Autocatalysis txiav nws tus kheej, yog li tsim cov nquag pepsin. Tom qab ntawd, pepsin generated txuas ntxiv mus txiav pepsinogen, txiav tawm 44 amino acid residues thiab tsim ntau pepsin. Qhov no mechanism ntawm kev tswj cov ntaub ntawv ntawm zymogen thaum tsis muaj zaub mov digestion tiv thaiv ntau pepsin los ntawm digesting lub plab phab ntsa nws tus kheej, uas yog ib tug tiv thaiv mechanism.

Khoom Specification

Pepsin Extract yog dab tsi?

Muaj ob txoj kev rho tawm tseem ceeb ntawm pepsin: acid extraction thiab alkali extraction.

Ntawm lawv, acid extraction yog qhov yooj yim, tab sis lub tshuab txais pepsin tau muab rho tawm, uas muaj kev ruaj ntseg qis. Cov pepsinogen uas tsis tau ua haujlwm tau muab rho tawm los ntawm txoj kev alkaline, thiab cov protein tau txais yog qhov ruaj khov, tab sis lub sijhawm rho tawm yog ntev thiab muaj ntau cov protein ntau.
Txawm li cas los xij, feem ntau ntawm cov paub pepsin tau muab rho tawm los ntawm txoj kev alkaline, uas yog qhov zoo rau kev kawm txog kev ua kom muaj zog ntawm zymogen thiab nws cov kev khaws cia ntev.
Tam sim no, cov txheej txheem paub tab rau kev sib cais thiab purifying pepsin yog raws li nram no: 1. Leaching; 2. salting tawm thiab nag lossis daus, uas tuaj yeem ntxiv los ntawm txoj kev sib cais membrane; 3. Ion pauv chromatography thiab gel filtration chromatography. Cov tshuaj enzyme crude yog feem ntau sib cais los ntawm salting-tawm los nag txoj kev, ntxiv los ntawm ultrafiltration membrane sib cais kom tshem tawm ntsev thiab impurities feem ntau, thiab tib lub sij hawm, lub hom phiaj ntawm concentration yuav ua tiav. Tom ntej no, cov txheej txheem ntawm kev sib txuas gel kem thiab ion txauv kem tau siv rau kev ua kom huv ntxiv, thiab purity ntawm pepsin tuaj yeem siab dua 95%.

Tus cwj pwm atlas ntawm Pepsin

Infrared spectrum ir1 ntawm Pepsin

Infrared spectrum ir2 ntawm Pepsin

Dab tsi yog qhov kev npaj ntawm pepsin (los ntawm npua)?

Grate yaj thiab bovine testis, extract acetic acid thiab hydrochloric acid, thiab salting tawm ammonium sulfate los ntawm theem.

Secondary salting tawm, ammonium sulfate dialysis, buffer tov kom tshem tawm cov cua sov, sodium phosphate, calcium acetate

Lim thiab qhuav cov khoom tiav.

Cov yaj thiab cov nyuj khov tau raug txiav nrog rab riam, thiab sab hauv thiab sab nraud thiab cov khoom siv testis raug stripped thiab hauv av rau hauv chyme. Qhov hnyav 100kg ntawm slurry thiab ncuav nws mus rau hauv lub npaj txias acetic acid tov (9{54}}kg dej, 600ml ntawm glacial acetic acid, thiab 10L ntawm 1mol / L hydrochloric acid), do nruj ntawm -5 degree rau 3 ~ 4 min, ces do txhua 5 feeb thiab muab rho tawm rau 4 h .. ces lim kom tau 125-140 l ntawm extract. Lub pulp residue yog muab rho tawm rau lub sij hawm thib ob, thiab tus nqi ntawm cov kua qaub muab rho tawm yog 1/3 ntawm thawj zaug, thiab cov kev daws teeb meem sib xyaw ua ke. Nyob rau hauv tas li stirring, 210g ntawm ammonium sulfate tau ntxiv rau txhua liter ntawm cov extract, thiab tom qab nws tag nrho yaj, nws raug tso cai sawv ntsug li 1 teev, thiab lim ib hmos nrog polyester ntaub kom tau 110L ntawm filtrate. Tom qab ntawd, 290g ntawm ammonium sulfate tau ntxiv rau txhua liter ntawm lim kom yaj tag, thiab tom qab ntawd nws raug tso cai sawv ntsug rau 1 teev thiab lim thaum hmo ntuj kom tau txais hyaluronidase crude. Dissolve cov khoom crude nyob rau hauv 5L txias distilled dej, ntxiv 125g ammonium sulfate nyob rau hauv nplawm kom tag yaj nws, thiab tawm ntawm 10 degree ib hmos. Tshem tawm cov rog ntawm cov kua dej, siphon tawm lub supernatant thiab lim. Cov filtrate tau nplawm tas li, thiab tom qab ntawd 750g ntawm ammonium sulfate tau maj mam ntxiv kom yaj tag nrho, thiab nws tau tso tseg thaum hmo ntuj ntawm txog 10 degree. Hnub tom qab, tus supernatant tau nqus kom tau cov khoom qab ntsev. Dissolve cov ntsev tawm hauv 500ml dej txias distilled, muab tso rau hauv lub hnab lim ntshav, muab tso rau hauv 25L phosphate citric acid buffer tov nrog pH=65 (citric acid 75g, Na2HPO4 12H2O 60g, sodium chloride. 70g, ua los ntawm dej mus rau 2500ml, kho nrog citric acid rau pH =6.5), dialyze ntawm li 10 degree rau 24h, thiab lim kom tau dialysate. Txias lub dialysate hauv ib da dej dej khov, ntxiv 50ml ntawm 15% Na2HPO4 · 12H2O tov, maj mam ntxiv 30ml ntawm 20% calcium acetate monohydrate tov nyob rau hauv tas li stirring, kho tus nqi pH rau 8.5 nrog 0.5mol / L sodium hydroxide tov, txuas ntxiv stirring 10min, lim hauv qhov txo qis, kho tus nqi pH ntawm cov lim mus rau 7.0 nrog 0.5 mol / L hydrochloric acid, thiab khov-qhuav kom tau cov iav tsis muaj pyrogen. Cov txiaj ntsig yog ntau tshaj 2 × 104 units ib kilogram ntawm testis.

Pepsin siv qhov twg?

Kev lag luam pepsin yog muab rho tawm los ntawm glandular txheej ntawm npua plab. Nws yog ib feem ntawm rennet, uas yog siv los coagulate mis nyuj thaum lub sij hawm tsim cheese. Pepsin muaj ntau daim ntawv thov hauv kev tsim khoom noj: hloov pauv cov taum pauv protein thiab gelatin los muab whipping zoo, [40] hloov cov zaub protein rau cov khoom noj txom ncauj uas tsis yog mis nyuj, ua cov khoom noj ua ntej rau hauv cov cereal kub tam sim, [41] thiab npaj tsiaj thiab zaub protein. hydrolysates rau flavoring zaub mov thiab dej haus. Hauv kev lag luam tawv, nws yog siv los tshem tawm cov plaub hau thiab cov ntaub so ntswg los ntawm qhov nkaum, thiab kom rov qab tau nyiaj los ntawm cov duab thaij duab los ntawm kev zom cov gelatin txheej uas muaj nyiaj. [42] Keeb kwm, pepsin yog ib qho ntxiv ntawm Beaman zom cov pos hniav uas tsim los ntawm Dr. Edwin E. Beaman.

Yuav ua li cas thiab qhov twg yuav pepsinhmoovlag luam wholesale?

Koj tuaj yeem tso siab rau peb qhov kev txiav txim, vim tias peb muaj cov ntaub ntawv zoo ntawm kev muag tshuaj raws cai.

Peb ua qhov kev kuaj xyuas ywj pheej ntawm txhua yam khoom thiab tom qab ntawd muag rau peb cov neeg siv khoom hwm.

Xa koj Cov Lus Nug kom paub meej ntxiv, Nyem email:allen@faithfulbio.comTam sim no.

Muaj ntau txoj kev xa khoom rau koj xaiv

Xa koj Cov Lus Nug kom paub meej ntxiv, Nyem email:allen@faithfulbio.comTam sim no.

Tsis lees paub: Cov ntawv luam tawm ntawm lub xaib no yog siv los txhawb kev sib pauv kev kawm thiab kev kawm, thiab tsis txhais hais tias lub vev xaib no pom zoo nrog nws cov kev xav lossis lees paub qhov tseeb ntawm nws cov ntsiab lus. Thov lim kom zoo. Yog tias koj ntseeg tias tsab xov xwm no ua txhaum, thov hu rau tus thawj tswj hwm. Peb tsis muaj lub luag haujlwm rau qhov tshwm sim ntawm kev siv cov ntaub ntawv thiab cov kev pabcuam hauv lub vev xaib no.

Nco tseg: Cov tshuaj no yuav tsum tau siv rau kev tshawb fawb nkaus xwb. Cov lus thov no tsis tau raug soj ntsuam los ntawm Food and Drug Administration. Thov nrog koj tus kws kho mob tham thiab kawm txog cov kev tshawb fawb muaj ua ntej siv lawv. Cov khoom no tsis yog tsim los kuaj xyuas, kho, kho lossis tiv thaiv kab mob.