Trans Zeatin yog lub ntuj adenine derivative thiab feem ntau lom cytokinin isomer. Nws tso siab rau cov purine core thiab cov trans-isopentenyl hydroxyl sab saw kom ua tiav cov neeg txais kev lees paub thiab kho ob pawg ntawm cov teeb liab teeb liab hauv cov nroj tsuag. High-purity Trans Zeatin yog synthesized los yog lawm muab rho tawm thiab refined. Cov cis-isomer impurities thiab oxidative degradation fragments raug tswj nruj me ntsis. Nws yog dav siv nyob rau hauv cog cov ntaub so ntswg kab lis kev cai, lub cev sib txawv, nplooj senescence kev cai, thiab cog kev nyuaj siab kuj physiological kev taw qhia.Trans Zeatintsom rau kev tsav tsheb cytoplasmic division, tsim kom muaj kev sib cuam tshuam nrog auxin, uas sib koom ua ke txiav txim siab ntawm callus cag thiab tua sib txawv. Nws yog ib qho kev lees paub zoo tshaj plaws hauv kev soj ntsuam cov khoom siv tshuaj hormones cog.
🧪 Sab saw configuration txiav txim siab molecular paub thiab solubility.
Trans Zeatin's molecular skeleton yog ua raws li lub nplhaib adenine purine ntawm nws cov tub ntxhais, nrog cov saw hlau sab hauv hydroxyisoprene uas muaj ob daim ntawv cog lus ntawm N6 qhov chaw. Qhov kev npaj ntawm trans spatial no yog qhov tseem ceeb rau kev ua kom muaj bioactivity siab; ib tug cis configuration nrog spatial twisting ho txo nws receptor binding peev xwm. Tag nrho cov molecule muaj ob pawg polar hydroxyl thiab hydrophobic olefin carbon saw, tso cai rau kev sib cais thiab kev sib cais hauv cov kua qaub tsis muaj zog aqueous thiab cov nroj tsuag kab lis kev cai media solvents yam tsis muaj nag lossis daus loj. Nyob rau hauv loj - nplai callus kab lis kev cai thiab explant tshuaj xa tshuaj ntsuam xyuas tshuab, nws ua kom zoo ib yam zoo molecule concentration nyob rau hauv txawv kab lis kev cai hlab ntsha, txo cov kev loj hlob cov ntaub ntawv hloov pauv los ntawm solubility sib txawv. Lub nplhaib purine conjugated yog tshuaj lom neeg ruaj khov thiab tsis yooj yim rau oxidation thiab lub nplhaib -qhib nyob rau hauv qis - kub, lub teeb - tiv thaiv cia tej yam kev mob. Npaj cov kev daws teeb meem tuaj yeem raug aliquoted thiab cryopreserved, txo lub nra hnyav dua.
Tom qab hu rau cov nroj tsuag cell membrane, cov molecule nkag mus rau hauv lub cell nrog kev pab los ntawm transmembrane thauj proteins, tsis muaj cuam tshuam cov phospholipid bilayer qauv. Ib zaug nyob rau hauv lub cell, Trans Zeatin tsis ua raws li indiscriminate tshuaj tiv thaiv; Feem ntau cov molecules tsis tu ncua tswj lawv qhov kev sib haum xeeb thiab mus rau lawv qhov chaw receptor protein nyob rau hauv endoplasmic reticulum membrane rau kev paub thiab kev khi. Cov hlwb cog muaj ib txoj hauv kev glycosylation uas hloov pauvTrans Zeatinrau hauv daim ntawv glycosylated cia. Thaum lub physiological ib puag ncig hloov pauv, daim ntawv khaws cia no tuaj yeem tso tawm cov active dawb molecule dua. Qhov kev hloov pauv hloov pauv no tuaj yeem tswj hwm cov txiaj ntsig zoo ntawm cov khoom siv molecules hauv lub xovtooj ntawm tes, tsis txhob muaj qhov hloov pauv loj hauv cov dawb molecule concentration thiab ntau ze ze rau qhov kev ua haujlwm tiag tiag ntawm cov tshuaj hormone endogenous turnover hauv cov nroj tsuag.
Trans Zeatin feem ntau lees paub histidine kinase receptors nyob rau ntawm cov qauv membrane, tsis tshua nkag mus rau hauv lub cell nucleus kom ncaj qha mus chromatin, thiab tsis ncaj qha khi rau DNA sequences los cuam tshuam cov noob replication. Txawm hais tias muaj ntau dua exogenous concentrations, nws tsis ua rau kev puas tsuaj xws li chromosome breakage los yog txawv txav genome rearrangement nyob rau hauv cov nroj tsuag hlwb. Nyob rau hauv ntev - lub sij hawm explant subculture systems, cov txheej txheem ntawm cell ciaj sia taus thiab cov txheej txheem metabolic tsis raug cuam tshuam rau qhov tsis muaj -kev cuam tshuam tshwj xeeb, tso cai rau kev soj ntsuam dawb huv ntawm phenotypic kev hloov pauv uas tshwm sim los ntawm kev faib tawm ntawm tes thiab kev sib txawv ntawm lub cev tom qab receptor activation, zam kev cuam tshuam los ntawm kev sib txawv ntawm cov noob caj noob ces.
Cov molecule reversibly khi rau histidine kinase receptor ntawm hydrogen bonds thiab hydrophobic stacking. Thaum intracellular free Trans Zeatin degraded los ntawm glycosidases los yog hloov mus rau ib qho chaw cia- khi lub xeev, cov molecule detaches los ntawm CHASE domain ntawm receptor protein, thiab cov receptor kinase kev ua tau raug kaw tam sim ntawd, nrog downstream phosphate hloov chaw pib maj mam rov qab mus rau theem pib. Cov txheej txheem no tsis ua rau tsis tu ncua irreversible receptor activation, thiab nws tsis induce compensatory overexpression ntawm receptor protein, thiab nws tsis ua rau lub hom phiaj desensitization. Nyob rau hauv vitro system nrog alternating kev kho mob ntawm exogenous cov tshuaj rho tawm, dynamic fluctuations ntawm endogenous cov tshuaj hormones nyob rau hauv cov nroj tsuag yuav replicated, tso cai rau cov kinetic cov ntaub ntawv ntawm cov nroj tsuag organogenesis kom ntau ze ze xav txog qhov tseeb nyob rau hauv lub xeev ntawm cov nroj tsuag nyob.
⚙️ Ib ob -cov khoom siv teeb liab pib ua rau hauv lub cellular phosphate cascade.
Thaum Trans Zeatin khi rau AHK tsev neeg histidine kinase receptor ntawm lub endoplasmic reticulum membrane, cov receptor protein tau hloov pauv, ua rau autophosphorylation ntawm histidine residues. Cov pab pawg phosphate yog tom qab ntawd thauj mus rau sab hauv los ntawm cytoplasmic phosphotransporters, thaum kawg nkag mus rau hauv lub nucleus thiab xa cov phosphate pawg mus rau hom B teb regulator. Hom B teb cov tswj hwm yog DNA-binding transcription activators; Tom qab tau txais kev hloov kho phosphate, lawv khi rau cov cheeb tsam txhawb nqa ntawm cov noob caj noob ces, pib qhov kev nthuav qhia ntawm ntau cov noob muaj feem xyuam rau lub voj voog ntawm tes, organogenesis, thiab kev ntxhov siab, ua tiav tag nrho cov teeb liab transduction txheej txheem los ntawm extracellular hormone signals rau nuclear gene qhia reprogramming.
Hom A cov neeg tswj xyuas cov lus teb yog cov lus qhia tsis zoo tseem ceeb hauv txoj hauv kev no. Kev ua kom cov hom B regulators ncaj qha induces lub synthesis ntawm loj npaum li cas ntawm hom A teb regulators. Thaum tau txais cov phosphate pab pawg neeg, hom A teb regulators, nyob rau hauv lem, inhibit upstream phosphate hloov, yog li txwv tsis pub siv lub teeb liab thiab tiv thaiv ntau amplification ntawm cytokinin signals. Qhov kev tawm tswv yim tsis zoo no pab cov nroj tsuag tsis cuam tshuam cov teeb liab poob siab los ntawm exogenous Trans Zeatin, tiv thaiv lawv los ntawm indiscriminately pib faib cov txheej txheem tsuas yog vim muaj zog exogenous molecule concentration. Nws tuav lub cell elasticity nyob rau hauv cov tshuaj hormones teb, thiab cov txheej txheem no yog ib qho tseem ceeb hauv paus ntsiab lus rau cov nroj tsuag kom hloov mus rau qhov sib ntxiv ntawm cov tshuaj hormones exogenous.
Qhov tseem ceeb physiological nyhuv ntawmTrans Zeatinyog tsav cytokinesis. Tom qab nuclear replication, cov txheej txheem cytokinesis xav tau cytokinin signaling. Intracellular teeb liab reprogramming upregulates cov kev qhia ntawm cyclin-cov noob caj noob ces, propelling lub cell los ntawm G2 theem mus rau theem M thiab ua kom lub cell division tag nrho. Auxin ib leeg tuaj yeem txhawb nqa cell elongation thiab nthuav dav, tab sis tsis tuaj yeem tsav cytokinesis zoo. Qhov no piav qhia vim li cas Trans Zeatin thiab auxin yuav tsum ua haujlwm ua ke hauv cov ntaub so ntswg kab lis kev cai kom ua tiav cov callus proliferation sai.
Qhov sib npaug ntawm qhov sib npaug ntawm cov tshuaj hormones txiav txim siab qhov sib txawv ntawm lub cev. Thaum tus txheeb ze ntawm Trans Zeatin nyob rau hauv cov kab lis kev cai nruab nrab yog siab dua, nws tsav callus sib txawv ntawm lub paj hlwb, inducing ib tug loj tus naj npawb ntawm adventitious buds; Nyob rau hauv tej yam kev mob uas auxin concentration yog dominant, nws induces hauv paus hauv paus tsim. Txawm tias me ntsis kev hloov pauv hauv qhov sib piv ntawm qhov sib piv ntawm ob cov tshuaj hormones tuaj yeem ua rau muaj qhov sib txawv ntawm qhov sib txawv ntawm kev cog qoob loo. Qhov kev sib cuam tshuam logic no khiav los ntawm tag nrho cov txheej txheem ntawm cov nroj tsuag hauv vitro regeneration. Los ntawm kev teeb tsa gradient piv, peb tuaj yeem soj ntsuam qhov kev loj hlob ntawm cov tua, cov hauv paus hniav, thiab cov ntaub so ntswg callus nyob rau hauv cov tshuaj hormones sib txawv thiab txheeb xyuas cov cai hauv qab ntawm tus ntoo khaub lig- kev tswj hwm ntawm ob lub xov tooj ntawm tes.
🔬 Ntau cov kev cai physiological reshape cog kev loj hlob thiab kev nyuaj siab ntev
Kev tshem tawm ntawm apical dominance yog ib qho kev tshwm sim ntawm lub cev ntawm Trans Zeatin. Auxin synthesized thiab exported los ntawm cov nroj tsuag apical bud yog thauj downwards, inhibiting kev loj hlob ntawm lateral buds. Trans Zeatin, synthesized nyob rau hauv cov hauv paus hniav thiab thauj upwards los ntawm lub xylem, mus txog rau lateral bud sites thiab antagonizes inhibitory nyhuv ntawm auxin, awakening dormant lateral buds thiab prompting lawv pib cell division thiab elongation, ua rau ntau branching. Cov txheej txheem no qhia tau hais tias cov hauv paus hniav thiab saum toj no - qhov chaw hauv av tuaj yeem ua tiav ntev - kev sib txuas lus ntawm lub cev los ntawm kev thauj mus los ntawm cov tshuaj hormones, kev sib koom ua ke tag nrho cov nroj tsuag architecture. Exogenous daim ntawv thov ntawm Trans Zeatin tuaj yeem simulate hauv paus- tau txais cov cim tshuaj hormones, ncaj qha saib kev loj hlob hloov tom qab lateral bud dormancy lawm, thiab elucidate cov tshuaj hormones kev sib cuam tshuam logic tom qab cog architecture tswj.
Nyob rau hauv cov xwm txheej kev ntxhov siab, Trans Zeatin tuaj yeem pab cov nroj tsuag tsis muaj kev puas tsuaj los ntawm qhov chaw tsis zoo. Tsis tshua muaj kub, salinity, thiab drought ceev cov tsub zuj zuj ntawm reactive oxygen hom nyob rau hauv cov nroj tsuag, cuam tshuam lub photosynthetic system qauv. Trans-Zeatin- mediated signaling networks upregulate the plant's antioxidant enzyme system, nce kev ua haujlwm ntawm superoxide dismutase thiab catalase, tshem tawm cov kab mob hauv cov cellular reactive oxygen ntau dhau, txo cov cell puas los ntawm lipid peroxidation, tswj chloroplast cov qauv kev ntseeg siab, thiab ua kom cov txheej txheem kev ua haujlwm tsis tu ncua. Hauv kev ntxhov siab- kho hauv vitro yub system, supplementation nrog exogenous Trans-Zeatin alleviated qhov poob ntawm photosynthetic efficiency thiab txhim kho cov yub ciaj sia, tag nrho ua rau pom cov tshuaj hormones-mediated physiological chain of stress resistance.
Trans-Zeatin kuj koom nrog hauv kev sib koom tes teb rau cov nroj tsuag cov zaub mov. Thaum nitrogen cov as-ham xws li nitrates txaus nyob rau hauv cov av, nws txhawb kev nce hauv cov synthesis ntawm Trans-Zeatin hauv cov hauv paus hniav. Tom qab kev sib xyaw ua ke, nws raug thauj mus rau qhov chaw saum toj kawg nkaus ntawm xylem, qhia cov qia thiab nplooj cov ntaub so ntswg los kho lawv txoj kev loj hlob kom haum rau qib ntawm cov khoom noj sab nraud. Thaum tsis tshua muaj nitrogen, theem ntawm cov tshuaj hormone synthesis txo qis raws li, thiab cov nroj tsuag txoj kev loj hlob qeeb qeeb. Cov tshuaj hormones ua raws li cov neeg xa xov, xa cov cim qhia txog cov txheej xwm hauv av rau saum toj no - cov kab mob hauv av, hla cov cim qhia ib puag ncig sab nraud thiab cov kev pabcuam hauv cov nroj tsuag. ExogenousTrans ZeatinKev kho mob tuaj yeem siv los qhia txog kev sib koom ua ke ntawm cov nroj tsuag kev loj hlob los ntawm cov as-ham thiab cov tshuaj hormones.
Thaum ntsib kev kis kab mob los ntawm qee yam kab mob, Trans Zeatin tuaj yeem txhim kho cov nroj tsuag tus kheej cov txheej txheem tiv thaiv. Tom qab receptor activation, nws tuaj yeem sib koom ua ke nrog txoj hauv kev tiv thaiv salicylic acid, ua rau muaj kev nthuav tawm ntawm cov kab mob - tiv thaiv - cov noob caj noob ces thiab txhim kho cov nroj tsuag tiv thaiv qee cov kab mob. Cov nyhuv no tsis ncaj qha tua cov kab mob, tab sis theej reprograms cov nroj tsuag intracellular tiv thaiv transcriptome, muab cov nroj tsuag muaj zog tiv thaiv. Nyob rau hauv vitro kuaj inoculated nrog pathogens, cov ntaub ntawv pretreated nrog Trans Zeatin pom ib tug tseem ceeb txo nyob rau hauv pathogen proliferation, uas yuav siv tau los elucidate txoj kev logic ntawm cov tshuaj hormones tswj kev tiv thaiv cov nroj tsuag.
📌 Cov cytokinins nquag tau hloov mus rau ntau -cov kev tshawb fawb cov nroj tsuag
Trans Zeatin yog cov cuab yeej siv tseem ceeb hauv kev tshawb fawb cytokinin, siv los sib piv cov haujlwm sib txawv ntawm lwm cov isomers thiab ntau yam hluavtaws cytokinins nyob rau hauv cov nqe lus ntawm receptor affinity, mitotic kev ua, tua sib txawv txhawb, thiab tiv thaiv -aging. Kev cia siab rau qhov ruaj khov purity thiab siab trans- feem pua kev teeb tsa ntawm Trans Zeatin raws li cov qauv ntsuas, qhov cuam tshuam ntawm sab- kev hloov kho saw thiab ob npaug- kev sib cog lus hloov pauv ntawm molecular physiological kev ua ub no tuaj yeem txheeb xyuas, pab rau kev nkag siab ntawm cov qauv siv - kev sib raug zoo ntawm kev tshuaj ntsuam ivatives thiab optimization ntawm cov qauv tshiab cog kev loj hlob regulatory molecules.
Nws tuaj yeem siv los tsim ntau yam hauv vitro cog kev ntsuam xyuas, nrog kev tswj hwm tshuaj gradient los simulate physiological scenarios xws li callus proliferation, explant organ regeneration, detached leaf senescence, seedling abiotic stress treatment, and seed dormancy release. Kev siv ntau txoj hauv kev xws li kev soj ntsuam morphology, ntsuas cov ntsiab lus chlorophyll, gene transcription quantification, cov ntaub so ntswg ntawm tes suav, thiab branching phenotypic cov txheeb cais, txoj kev tshawb no nthuav dav elucidates tag nrho cov saw ntawm Trans Zeatin, los ntawm transmembrane lees paub, receptors ua kom sib txawv, thiab photo activation. thiab kev nyuaj siab teb. Nws delineates zoo concentration ranges rau txawv nyob rau hauv vitro sim scenarios, accumulating nruj thiab ncauj lus kom ntxaws cov ntaub ntawv nyob rau hauv vitro rau txoj kev loj hlob ntawm cov nroj tsuag kab lis kev cai nruab nrab formulations.
Hauv peb-seem cog organoid thiab hauv vitro meristem kab lis kev cai kab lis kev cai, exogenous Trans Zeatin tuaj yeem nkag mus rau ntau txheej txheej ntawm cov nroj tsuag, simulating cov tshuaj hormone gradient faib nyob rau hauv cov nroj tsuag, kov yeej qhov kev txwv ntawm ob-dimensional explants nyob rau hauv replicating lub meristem microenvironment. Nws tso cai rau kev soj ntsuam tag nrho cov txheej txheem ntawm qia cell homeostasis txij nkawm thiab lub cev primordium pib nyob rau hauv txawv cov tshuaj hormones concentration gradients, txhim kho qhov tseeb ntawm cov ntaub ntawv hauv vitro kev sim hauv kev kwv yees hauv vivo cov txheej txheem kev loj hlob thiab kho cov txheej txheem kev ntsuam xyuas rau cov nroj tsuag cov tshuaj hormones ntawm lub cev.
Cov kev tshawb fawb reagents nthuav dav kev sib raug zoo, tso cai rau kev co- ua cov qauv nrog auxins, abscisic acid, gibberellins, thiab receptor inhibitors, tsim kom muaj kev ntsuam xyuas rau kev nkag mus rau sab saud thiab nqes hav validation. SivTrans Zeatinib leeg los ua kom tiav cytokinin signaling, ua ke nrog cov tshuaj hormones sib xws lossis txoj hauv kev inhibitors, tso cai rau kev txheeb xyuas kev sib koom ua ke lossis kev sib raug zoo ntawm txoj kev sib txawv hormonal. Qhov no qhia meej txog lub luag haujlwm tseem ceeb thiab theem nrab ntawm ntau lub hormonal tes hauj lwm thaum lub sij hawm kev loj hlob, muab nyob rau hauv - tsom xam qhov tob ntawm yuav ua li cas cov nroj tsuag cia siab rau cov synergistic los ntawm ntau yam tshuaj hormones los txiav txim cell destiny, zoo li cog cog architecture, thiab hloov mus rau kev ntxhov siab, yog li nthuav cov theoretical ciam teb ntawm cov nroj tsuag kev loj hlob biology.
Trans Zeatin residue nyob rau hauv cov dej khib nyiab yog ib qho me me purine molecule. Ib puag ncig microbial hydrolytic thiab oxidative enzymes tuaj yeem maj mam decompose lub nplhaib purine thiab isopentenyl sab saw, thaum kawg degrading nws mus rau carbon dioxide thiab nitrogenous inorganic ntsev. Nws tsis tsim kev pheej hmoo, recalcitrant organic pollutants, thiab cov txheej txheem kuaj biochemical cov dej khib nyiab tuaj yeem ua tiav kev pov tseg tsis zoo. Loj - kev tshawb fawb loj ntawm cov kab lis kev cai cog qoob loo, kev soj ntsuam cov nplooj tsis pom kev, kev kho cov nroj tsuag kev ntxhov siab, thiab kev txheeb xyuas cov tshuaj hormones tuaj yeem tshawb xyuas tag nrho cov txiaj ntsig ntawm cov cytokinins ntuj thaum txo qis ib puag ncig lub nra ntawm kev sim pov tseg, ntsuas kev tshawb fawb tshawb fawb thiab cov ntsiab lus ntsuab pov tseg.
Xaus
Trans zeatin yog ib qho ntawm cov tswv cuab tseem ceeb tshaj plaws ntawm tsev neeg cytokinin ntuj. Nws tswj cov cog cell faib, qeeb senescence, thiab tswj cov qia cell kev ua los ntawm activating histidine kinase receptor signaling txoj kev. Nws ua lub luag haujlwm tsis tuaj yeem hloov pauv raws li lub zog cytokinin hauv cov nroj tsuag kab lis kev cai thiab noob caj noob ces.
Xi'an Faithful BioTech Co., Ltd. siv cov cuab yeej siv siab heev thiab cov txheej txheem los xyuas kom meej - cov khoom zoo. PebTrans zeatinua tau raws li cov qauv tshuaj thoob ntiaj teb. Peb txoj kev nrhiav kev ua tau zoo, tus nqi tsim nyog, thiab cov kev pabcuam zoo tshaj plaws ua rau peb tus khub rau cov tsev kho mob thiab cov kws tshawb fawb thoob ntiaj teb. Yog tias koj xav tau Trans zeatin kev tshawb fawb lossis kev tsim khoom, thov hu rau peb Nyem email:allen@faithfulbio.comLos yog WhatsApp:+86 13137770562.
Cov ntaub ntawv
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- MedChemExpress. (nd). Trans-Zeatin (HY-N0575). Tau txais Lub Yim Hli 16, 2026.
- Inoue, T., et al. (2001). Kev txheeb xyuas ntawm CRE1 raws li cytokinin receptor los ntawm Arabidopsis. Xwm, 409(6823), 1060-1063.
- Hwang, I., et al. (2002). Cytokinin signaling networks. Kev Ntsuam Xyuas Txhua Xyoo ntawm Tsob Ntoo Biology, 53, 401-426.